A prospective PCR–based screening for the EML4-ALK oncogene in non-small cell lung cancer Running title: PCR–based screening for EML4-ALK
نویسندگان
چکیده
Division of Functional Genomics, Jichi Medical University, Tochigi 329-0498, Japan; Department of Respiratory Medicine, Toho University Omori Medical Center, Tokyo 143-8541, Japan; Tohoku University Hospital, Miyagi 980-8574, Japan; Miyagi Cancer Center, Miyagi 981-1293, Japan; First Department of Medicine, Hokkaido University School of Medicine, Hokkaido 060-8638, Japan; Asahikawa Medical Center, Hokkaido 070-8644, Japan; Nippon Medical School Hospital, Tokyo 113-8603, Japan; Pathology Project for Molecular Targets, The Cancer Institute, Tokyo 135-8550, Japan; Department of Medical Genomics, Graduate School of Medicine, University of Tokyo, Tokyo 113-0033, Japan; Saitama Medical University Hospital, Saitama 350-0495, Japan; CREST, Japan Science and Technology Agency, Saitama 332-0012, Japan
منابع مشابه
Imaging, Diagnosis, Prognosis A Prospective PCR-Based Screening for the EML4-ALK Oncogene in Non–Small Cell Lung Cancer
Purpose: EML4-ALK is a lung cancer oncogene, and ALK inhibitors showmarked therapeutic efficacy for tumors harboring this fusion gene. It remains unsettled, however, how the fusion gene should be detected in specimens other than formalin-fixed, paraffin-embedded tissue. We here tested whether reverse transcription PCR (RT-PCR)-based detection of EML4-ALK is a sensitive and reliable approach. Ex...
متن کاملA prospective PCR-based screening for the EML4-ALK oncogene in non-small cell lung cancer.
PURPOSE EML4-ALK is a lung cancer oncogene, and ALK inhibitors show marked therapeutic efficacy for tumors harboring this fusion gene. It remains unsettled, however, how the fusion gene should be detected in specimens other than formalin-fixed, paraffin-embedded tissue. We here tested whether reverse transcription PCR (RT-PCR)-based detection of EML4-ALK is a sensitive and reliable approach. ...
متن کاملMultiplex reverse transcription-PCR screening for EML4-ALK fusion transcripts.
PURPOSE EML4-ALK is a fusion-type protein tyrosine kinase that is generated by inv(2)(p21p23) in the genome of non-small cell lung cancer (NSCLC). To allow sensitive detection of EML4-ALK fusion transcripts, we have now developed a multiplex reverse transcription-PCR (RT-PCR) system that captures all in-frame fusions between the two genes. EXPERIMENTAL DESIGN Primers were designed to detect a...
متن کاملThe EML4-ALK oncogene: targeting an essential growth driver in human cancer
Targeting of essential growth drivers represents an ideal approach to cancer treatment. To identify such molecules in clinical specimens, we developed a highly sensitive functional screening system based on the preparation of retroviral cDNA expression libraries. By screening such a library of lung adenocarcinoma with a focus formation assay, we discovered the EML4-ALK fusion-type oncogene. A s...
متن کاملEML4-ALK fusion gene and efficacy of an ALK kinase inhibitor in lung cancer.
PURPOSE The EML4-ALK fusion gene has been detected in approximately 7% of Japanese non-small cell lung cancers (NSCLC). We determined the frequency of EML4-ALK in Caucasian NSCLC and in NSCLC cell lines. We also determined whether TAE684, a specific ALK kinase inhibitor, would inhibit the growth of EML4-ALK-containing cell lines in vitro and in vivo. EXPERIMENTAL DESIGN We screened 305 primar...
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